Fig. 2.

Determination of genotypes in opaque seeds of the TO and WO lines by nested PCR. The left figures show the site of Tos17 insertion and the position of the primer pairs. Horizontal half arrows show the binding sites for nested PCR primers used for genotype determination (T1FT2F, 1R2R, and 3F6F for the SSI gene, and T1RT2R, 5F6F, and 5R6R for the SSIIIa gene). Primer pairs are indicated below the photographs. ‘T1FT2F/1R2R’ means that the primer pair T1F/1R was used for the first PCR and T2F/2R for the second PCR. M, molecular markers. (This figure is available in colour at JXB online.)