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. 2011 Jul 5;62(14):4819–4831. doi: 10.1093/jxb/err125

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 3. — Native-PAGE/SS activity staining of self-pollinated developing endosperm of ss1ss1/SS3ass3a in the TO line (A) and SS1ss1/ss3ass3a in the WO line (B) and their chain length distribution. Fourteen developing endosperm samples were randomly chosen, and the zymogram pattern of native-PAGE/SS activity staining was compared with those of Nipponbare, the ss1 mutant (ΔSSI, e7), and the ss3a mutant (ΔSSIIIa, e1). SSIIIa (A) or SSI (B) activity bands in 14 developing endosperm samples were divided into two groups, namely higher or lower activity bands, respectively, compared with Nipponbare. Lane numbers with circles indicate endosperm having the same or a slightly lower SSIIIa (A) or SSI (B) activity band, respectively, than that of Nipponbare. Chain length distributions of endosperm starch of a typical lower or higher activity band (boxed lanes) were analysed (bold lines) and compared with those of ΔSSI (thin line) and ss1ss1/SS3ass3a (dotted line) (A), or ΔSSIIIa (thin line) and SS1ss1/ss3ass3a (dotted line) (B). (This figure is available in colour at JXB online.)