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. 2011 Jun 24;62(14):4903–4916. doi: 10.1093/jxb/err180

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — The effect of 50 mM cysteine, 50 mM histidine, 5 mM GSH and 5 mM DTT on MgATP-dependent Cd transport into everted plasma membranes (A) and tonoplasts (B) isolated from control (open squares) and Cd-stressed (filled squares) plants. Orthovanadate was introduced into the reactions at a final concentration of 0.1 mM (0.1VO4) or 1 mM (1VO4). All incubation media with tonoplast and plasma membranes contained 50 mM nitrate or 5 μM gramicidin, respectively. Cd was introduced into the incubation media at a final concentration of 5 μM. The measurement was performed with the use of AAS followed by a direct filtration assay. The presented values are means ±SD of three independent experiments with each experiment done in triplicate. Squares indicate a significant difference (P <0.05) between control and Cd-treated plants and asterisks indicate a significant difference (P <0.05) between Cd transport in the presence of cysteine, histidine, GSH or DTT and corresponding Cd transport measured without these chemicals in the incubation media.