Table 1.

Effect of 0.02% Triton X-100 on the hydrolytic ATPase activity of V-ATPase and PM H⁺-ATPase in the fraction 2 enriched in tonoplast membranes. Enzyme activity was calculated from a difference between the amount of Pi produced in and out of presence of specific inhibitors (nitrate for V-ATPase or orthovanadate for PM H⁺-ATPase). The values presented are average with S.E. of three replications.

Detergent	V-ATPase hydrolytic activitya	PM H+ATPase hydrolytic activitya
+ Triton X-100	90 ± 4.2	25 ± 3.9
- Triton X-100	68 ± 5.3	6.3 ± 1.9

^aμg Pi mg^-1 protein h^-1