Table 2.
Effect of 0.02% Triton X-100 and 0.05% Brij 58 on the hydrolytic ATPase activity of PM H+-ATPase in the upper phase (fraction 1) of the PEG/DEX two-phase system enriched in plasma membranes. Enzyme activity was calculated from a difference between the amount of Pi produced in and out of presence of orthovanadate. The values presented are average with S.E. of three replications.
| Detergent | PM H+ATPase hydrolytic activitya |
| + Triton X-100 | 110.6 ± 3.9 |
| + Brij 58 | 115.36 ± 4.6 |
| -Triton X-100/- Brij 58 | 11.5 ± 1.3 |
a
μg Pi mg-1 protein h-1