Table 3.
Total content of ascorbate (ASC+DHA) (TA), ratio of ASC to DHA (ASC/DHA), total content of glutathione (GSH+GSSG) (TG), ratio of GSH to GSSG (GSH/GSSG), free amino acids (FAAs), and content of non-protein thiols (NP-SHs) in P. yunnanensis females and males, as affected by Cd, N deposition, and their combination, Fs, sex effect; Fm, Cd effect; Fn, N deposition effect; Fs×Fm, the interactive effect of sex and Cd; Fs×Fn, the interactive effect of sex and N deposition; Fm×Fn, the interactive effect of Cd and N deposition; Fs×Fm×Fn, the interactive effect of sex, Cd, and N deposition.
N deposition (g N m−2 year−1) | CdCl2·2.5H2O (mg kg−1 dry soil) | Sex | TA (μmol g−1 FW) | ASC/DHA | TG (nmol g−1 FW) | GSH/GSSG | FAAs (mg g−1 FW) | NP-SHs (μmol g−1 FW) |
0 | 0 | Female | 11.53±0.28 c | 7.30±0.33 a | 247.02±11.43 d | 1.62±0.06 a | 0.27±0.02 d | 0.35±0.02 d |
0 | 0 | Male | 13.20±0.47 abc | 4.30±0.21 c | 268.48±12.89 d | 1.24±0.05 b | 0.41±0.02 d | 0.42±0.02 cd |
0 | 50 | Female | 8.09±0.12 d | 2.93±0.11 de | 329.53±10.04 bc | 1.07±0.05 bc | 0.39±0.03 d | 0.62±0.03 b |
0 | 50 | Male | 11.73±0.50 c | 2.25±0.16 e | 392.95±13.53 a | 0.97±0.04 c | 0.58±0.02 c | 0.80±0.06 a |
6 | 0 | Female | 12.66±0.48 abc | 5.94±0.14 b | 298.18±8.73 cd | 1.55±0.05 a | 0.70±0.03 c | 0.43±0.02 cd |
6 | 0 | Male | 14.35±0.21 a | 4.08±0.23 c | 285.34±9.42 cd | 1.26±0.04 b | 1.26±0.03 a | 0.41±0.02 cd |
6 | 50 | Female | 11.88±0.49 bc | 4.38±0.28 c | 339.43±11.55 abc | 1.24±0.06 b | 0.86±0.02 b | 0.54±0.04 bc |
6 | 50 | Male | 13.77±0.51 ab | 3.66±0.11 cd | 383.76±10.36 ab | 1.13±0.03 bc | 0.96±0.04 b | 0.69±0.04 ab |
P: Fs | *** | *** | ** | *** | *** | *** | ||
P: Fm | *** | *** | *** | *** | NS | *** | ||
P: Fn | *** | * | * | * | *** | NS | ||
P: Fs×Fm | NS | *** | ** | ** | *** | ** | ||
P: Fs×Fn | NS | NS | NS | NS | *** | NS | ||
P: Fm×Fn | ** | *** | * | * | *** | ** | ||
P: Fs×Fm×Fn | NS | NS | NS | NS | *** | NS |
Each value is the mean ±SE (n=5). Values followed by the same letter in the same column are not significantly different according to Tukey's test. NS, not significant; *P <0.05; **0.01 ≤P <0.001; and ***P ≤ 0.001.