Fig. 2.

Transcript abundances in whole seeds of the three Arabidopsis thaliana GID1 genes during germination. (A) The time-course of endosperm rupture of A. thaliana (Col) seeds on medium without (CON) or with 10 μM GA₄₊₇ (GA) or 1 μM ABA added. Afterripened seeds were incubated at 24 °C in continuous light without preceding stratification. Mean values ±SE of 3×50 seeds. (B) In silico analysis on GID1 transcript abundance during early germination of unstratified Arabidopsis (Col) seeds based on eFP-Browser microarray data; Relative expression values ±SD for AtGID1a (At3g05120), AtGID1b (At3g63010), and AtGID1c (At5g27320). (C) GID1 transcript abundance pattern determined by qRT-PCR in whole seeds of Arabidopsis (Col) at 30 h, i.e. at 0% endosperm rupture for CON (see A). ddCt expression values relative to validated constitutive transcripts are presented. Medium additions as in (A). Mean values ±SE of 4× >1000 seeds.