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Subcellular localization of CSLD4 in vivo. GFP–CSLD4 (green) co-localized with RPA–DsRed2 (magenta) in pollen grains before germination (A). GFP–CSLD4 signals were also observed at the cell periphery at the future pollen tube exit site (B, arrow) and at the plasma membrane of the freshly emerged pollen tube tip (C, arrow) and of growing pollen tubes (E, arrow). GFP–CSLD4 signals were also found at the plasma membrane adjacent to pollen tube plugs (arrow). Bars: 5 μm.
