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. Author manuscript; available in PMC: 2011 Oct 14.
Published in final edited form as: Mol Cancer Res. 2009 Oct 13;7(10):1693–1703. doi: 10.1158/1541-7786.MCR-09-0031

FIGURE 6.

FIGURE 6

Inhibition of GATA-1 derepresses PU.1 target genes and inhibition of PU.1 derepresses GATA-1 targets in MEL cells. A. MELPUER cells (8 × 104) were transfected either with siRNA oligos inhibiting GATA-1 (si) or with negative control oligo (CO) using Lipofectamine. The cells were cultured either in the absence (CO) or in the presence of 10−7 mol/L of 17β-estradiol (+E). B. MEL cells (8 × 104) were transfected either with siRNA oligos inhibiting PU.1 (si) or with negative control oligo (CO) using Lipofectamine and cultured for 72 h. Total RNA was purified and subjected to quantitative reverse transcription-PCR as described in Materials and Methods. Y-axis, mRNA expression of indicated genes relative to housekeeping gene Hprt1. Bars, SE of two independent experiments.