Figure 4.

Inhibition of NF-κB translocation. NF-κB translocation in Human oesophageal squamous cells after 60-min exposure to pH 7.4 or pH 4. The top panels show cells that were pretreated for 1 h with the NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC) and incubated with PDTC during acid exposure, whilst the bottom panels did not receive PDTC treatment. NF-κB is detected in the nucleus of cells exposed to pH 4 in the absence of PDTC (arrows); however, in the presence of the inhibitor, NF-κB is located in the cytoplasm. NF-κB is also located in the cytoplasm of cells exposed to pH 7.4 with or without PDTC.