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. 2011 Oct;92(5):345–356. doi: 10.1111/j.1365-2613.2011.00778.x

Figure 6.

Figure 6

The effect of Human oesophageal squamous (HOS) conditioned medium on human oesophageal fibroblasts (HOF) cells using patient set 1 cells. NF-κB translocation in HOF cells following exposure to conditioned medium taken from HOS cells after treatment with medium at pH 7.4 or pH 5. Conditioned medium was taken after the following treatments: three 3-min treatments with a 5-min interval, three 3-min treatments with a 30-min interval or 30 min. Positive and negative controls are also shown. NF-κB translocation to the nucleus is observed in the cells treated with conditioned medium from cells exposed to pH 5 for 3 min with either a 5- or 30-min interval. Translocation is also observed in the positive control, but in all other cells, NF-κB remains in the cytoplasm.