Figure 4.
Evaluation of thermal stability by binding activity. SdAb A3 at 10 μg/ml and antibodies (llama anti-SEB IgG1, RA-SEB, and anti-SEB MAb 3b2a) at 100 μg/ml were heated at 85°C. Aliquots were removed and tested. The samples were diluted 10-fold and tested for their antigen binding ability in a Luminex direct binding assay.