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Comparison of CD spectra and intrinsic fluorescence intensity of sdAb A3 to llama IgG1 taken at 5°C intervals from 25°C to 85°C. SdAb A3 a) CD spectra b) fluorescence intensity. Llama anti SEB IgG1 c) CD spectra d) fluorescence intensity. Results indicate that intrinsic fluorescence changes fail to correlate with the sdAb's loss of ellipticity upon heating, while intrinsic fluorescence does correlate with ellipticity for the polyclonal IgG.
