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. 2011 Oct 3;108(41):17111–17116. doi: 10.1073/pnas.1108121108

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Fig. 2. — Analysis of MDSCs and TCR ζ-chain in tumors and lymphatic organs of transgenic mice. Cells from mice with (ret tu) or without (ret) macroscopic tumors and nontransgenic littermates (wt) were assessed by flow cytometry. (A) A representative dot plot of primary tumor is shown. (B) The weight of each tumor sample was plotted against the percentage of tumor-infiltrating Gr1⁺CD11b⁺ MDSCs within CD45.2⁺ leukocytes. (C) The duration of tumor progression was plotted against the percentage of MDSCs among live cells. The correlation was calculated by a linear regression analysis. (D) Cumulative data for MDSCs in metastatic LNs are expressed as the percentage of leukocytes (mean and SE from 10–31 mice). (E) ζ-Chain level in CD3⁺CD4⁺ TILs expressed as mean florescence intensity (MFI) and plotted against the tumor weight (n = 12). The correlation was analyzed by using a linear regression analysis. (F) Cumulative data for ζ-chain expression in metastatic LNs are shown as MFI (mean and SE; n = 5–12 mice per group; *P < 0.05).