Fig. 2.

Screening of potential diapause prevention agents indicates that three peptide analogs, 2Abf-Suc-FTPRLa (PK-2Abf), 2Abf-Suc-FKPRLa (DH-2Abf-K), and 2Abf-Suc-FVPRLa, are capable of preventing diapause (A) but that PK-2Abf also causes significant mortality (B). DH was tested at a dose of 1 nmol, whereas the other compounds were tested at a dose of 100 pmol. (C) Dose–response curve for DH-2Abf-K (structure in Fig. 1), the most potent agonist for preventing diapause. Error bars indicate SDs from three replicates; 12–20 larvae were tested in each replicate. The ED₅₀ was ∼7 pmol per larva. Differences between the tested compound and distilled water are shown by asterisks (Student t test: *P < 0.05; ***P < 0.001).