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. 2011 Sep 26;108(41):17105–17110. doi: 10.1073/pnas.1109095108

Fig. 3.

Fig. 3.

Cell-intrinsic requirement for Med1 in iNKT cell development. Flow cytometric analysis of iNKT cells in irradiated CD45.1+ congenic recipient mice reconstituted with 1:1 mixture of CD45.1+ wild-type and CD45.2+Med1+/− or CD45.2+ Med1−/− bone-marrow cells. The mice were analyzed 10 to 12 wk after bone marrow reconstitution. (A) Representative flow cytometric plots of iNKT cells derived from CD45.2+Med1+/− (Upper) or CD45.2+Med1−/− (Lower) donors in the bone-marrow chimeras. iNKT cells in the thymus, spleen, and liver were identified by CD1d-tetramers. (B and C) The frequency and absolute numbers of iNKT cells derived from CD45.2+Med1+/− or CD45.2+Med1−/− donor cells in the bone-marrow chimeras. Three mice from each group were analyzed.