Fig. 1.

Immune complex formation between endogenous DLC1 and talin in human cell lines. (A) Schematic representation of talin and DLC1 proteins and identified yeast two-hybrid (Y2H) interaction between a central region (residues 1,288–1,646) in the talin rod and DLC1 (residues 260–630). (B) Immune complex formation between endogenous talin and DLC1 in human cells was analyzed by reciprocal immunoprecipitation in 1634 fibroblasts and H1703 NSCLC cells (B Left) and human skin fibroblast line and H1703 (B Right). (C) Colocalization of endogenous DLC1 with talin; 1634 cells were stained with anti-talin (red) and anti-DLC1 (green) antibodies. The confocal images are representative of the majority of cells. (Scale bar: 5 μm.)