Fig. 3.

HS7 deletion negatively affects the expression of Pcdhαs, including PcdhαC2, in a subset of brain tissues. Total mRNA was extracted from the whole brain, cerebellum, cortex, hippocampus, and olfactory bulb of HS7 KO homozygous mice and 129Sv/Jae wild-type mice. Data for cortex, hippocampus, and olfactory bulb are included in SI Materials and Methods. Expression of individual Pcdhα isoforms was analyzed by qRT-PCR and normalized to Rps17. Each bar represents the average of at least three replicates and error bars represent SEM. Quantification of constant exon 3 (con3) represents total Pcdhα levels. *P < 0.05 comparing KO to wild-type expression using a two-tailed, unpaired Student t test.