Figure 1.

Elevated FVIII shortens the TTO after 2-minute, but not 3-minute, FeCl₃ injury to the carotid artery. C57Bl/6 mice were infused with saline or FVIII to 285% of normal. Thrombosis was induced by 10% FeCl₃ application to the carotid artery for the indicated time (3 or 2 minutes), and the TTO was determined by flow probe. In vessels that did not occlude, the TTO was recorded as 45 minutes. (A-E) Representative flow tracings following 3- (A-B) or 2-minute (C-E) FeCl₃ application to control (saline-infused, A,C,D) and FVIII-infused (B,E) mice. Gray shaded area represents time of vessel drying, FeCl₃ administration, and vessel washing, during which flow could not be monitored. Brackets indicate the time from FeCl₃ placement to the time of vessel occlusion. (F) Quantification of TTO data. Each point represents a separate mouse. Lines show median values to accommodate censored data at 45 minutes. *P < .05.