Fig. 2.

Phenotypic analysis of dfoxo^Δ94 homozygous mutant flies. (A) Egg-to-adult development time is delayed in both males and females homozygous for the dfoxo^Δ94 deletion (dfoxo−) compared with wDahomey controls. Only the eclosion period of the adult flies is shown. Data are shown as percentage of flies eclosing. (For males, n = 114 for wDahomey and n = 106 for dfoxo−. For females, n = 107 for wDahomey and n = 116 for dfoxo−). (B) Homozygous dfoxo^Δ94 flies (dfoxo−) have significantly reduced adult body weights and smaller wing sizes than wDahomey controls. Data are represented as means ± SEM (n = 10 for each measurement, **P <0.05, anova). (C) Clonal analysis of the effects of dfoxo^Δ94 mutation on cell size and proliferation in the developing wing disk. dfoxo^Δ94 mutant cells were generated by mitotic recombination and are marked by the absence of GFP. No obvious differences were observed in clone size nor in the size of in dfoxo^Δ94 mutant cells within the clone (GFP-negative cells) compared with adjacent heterozygous cells (GFP-positive cells) outside of the clone. (D) Survival curves of female flies homozygous mutant for dfoxo− mutants (n = 99, median survival = 41 days, maximum survival = 48 days) and wDahomey controls (n = 94, median survival = 61 days, maximum survival = 73 days). dfoxo− mutants are significantly shorter lived than controls (P <0.0001; Log-rank test). Representative of two independent experiments. (E) Average number of eggs laid per dfoxo− mutant 7-day-old female compared with age-matched wDahomey controls. Data are presented as the mean number of eggs laid per female over a 24-h period ± SEM. Eggs were counted from ten separate vials, and each vial contained ten females. dfoxo− females laid significantly fewer eggs than wDahomey controls. (**P <0.05, anova).