Table 1.
Percentage of differentiated cells positive for a certain marker [control vs. PDGF-AA + bpV(pic)] (Mean ± SEM) | |||
---|---|---|---|
Differentiation conditions | Condition A | Condition B | Condition C |
Marker | |||
β-III-tubulin | 2.9 ± 0.9 vs. 1.8 ± 0.3 | 7.4 ± 1.3 vs. 12.3 ± 2.4 | N/A |
α-SMA | 12.9 ± 2.8 vs. 18.4 ± 6.7 | 7.9 ± 1.6 vs. 7.9 ± 1.8 | N/A |
S100 | Rare | 13.2 ± 0.4 vs. 14.9 ± 2.7 | N/A |
Oil-red O | Rare | N/A | 35.7 ± 3.8 vs. 35.6 ± 2.1 |
None of the pairs of data showed statistically significant difference (i.e. P > 0.05 in t-test).
Condition A: DMEM/F12 with 1% fetal bovine serum (FBS).
Condition B: Neurobasal medium with 1% N2 supplement, 4 μm forskolin, 10 ng mL−1 heregulin and 1% FBS.
Condition C: DMEM/F12 with 1 mm dexamethasone, 1 mm isobutylmethylxanthine, 20 μg mL−1 insulin and 10% FBS.
N/A, not applicable; α-SMA, smooth muscle alpha actin.