Figure 7.

Cdc13 proteins employ either the N-terminal OB1 or the C-terminal OB4 domain for dimerization. (A, B, D) Self-association of each OB fold of KlCdc13 (A), CgCdc13 (B), and CaCdc13 (D) was examined in yeast two-hybrid assays. The color scheme of the OB folds is the same as in Figure 1. Self-association was reflected by the level of β-galactosidase activity produced by the reporter gene. Data are averages of three independent β-galactosidase measurements normalized to the dimeric interaction of the OB1 domain of ScCdc13 shown in Supplementary information, Figure S2C, arbitrarily set to 100. (C) Superposed gel-filtration profiles of ScCdc13_OB1, KlCdc13_OB1, CgCdc13_OB4, and CgCdc13_OB1.