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. 2011 Jul 12;21(9):1332–1342. doi: 10.1038/cr.2011.113

Figure 1.

Figure 1

The presence of immunochemically detectable 5-hydroxymethylcytosine (5-hmC) levels correlates with pluripotency in mammalian cells. 5-hmC is detectable in mouse and human (RH1 and RCM1) ESCs. (A) Lines stained with 5-hmC antibody. (B) 5-hmC and 5-mC staining of mouse ESC colonies (defined morphologically on the basis of phase images and indicated by dotted lines) and surrounding fibroblast-like differentiating cells. 5-hmC Immunostaining (C) and quantitative transcriptome deep sequencing (Solexa) data for Oct4, Nanog, Tet1, 2 and 3 (D) of RCM1 cells differentiating towards hepatic endoderm. Identical exposure times were used for different time points. Days post-induction are shown. RPKM – reads per kilobase per million reads. (E) High levels of 5-hmC are detectable in human iPSCs but not in human dermal fibroblasts (HDFs). DNA signal is indicated. Scale bars are 20 μm in A and E, 50 μm in B and 100 μm in C. The experiments were performed using the Diagenode antibody.