Figure 2.

5-Hydroxymethylcytosine (5-hmC) distribution in mouse pre-implantation development. (A) Distribution of 5-hmC and 5-mC during indicated pronuclear stages in mouse zygote. Merged and phase views, where pronuclei are distinguishable at PN₃-PN₄ stages, are shown. The exposure times were identical for each channel. 5-hmC antibodies produced by Active Motif were used. Scale bar, 10 μm. (B) 5-hmC Distribution at the indicated later stages of mouse pre-implantation development. 5-hmC Antibodies produced by Active Motif were used. 5-mC signal and merge view are indicated. ICM is arrowed. Scale bar, 10 μm.