Figure 4.
Patched1-binding ability and activity of three BDA1 mutant IhhN proteins. (A) Binding of altered IhhN proteins to the PTC1 receptor. Binding of 32P-IhhN to EcR-CHO cells stably expressing Ptc-CTD140 is measured in the presence of unlabeled recombinant proteins; binding is normalized to the amount (100%) bound in the absence of any competitors. (B) Alkaline phosphatase expression is analyzed in C3H10T1/2 cells after a 5-day incubation with purified WT IhhN protein or three mutants at 500 or 750 nM.