. 2011 May 3;21(9):1343–1357. doi: 10.1038/cr.2011.76

Table 1. Properties of mutant IhhN proteins.

Protein	Autoprocessing	Stability	Cholesterol modification	Palmitoylation	Multimer formation	Relative AKP induction activity at 500 nM (750 nM) in C3H10T1/2 assay	Dissociation constant (Kd) for Ptc-CTD, nM*	Heparin-binding affinity, salt elution concentration, M
WT	+	+++	+	+	+	1.0 (1.0)	20.6	0.32-0.49
E95K	+	+	+	+	+	0.47 (0.5)	40.6WΩ^※	0.32-0.66
E131K	+	+++	+	+	+	0.50 (0.52)	30.5	0.32-0.49
D100E	+	−	+	ND	ND	0.44 (0.38)	>100	ND

ND, not determined.

^*Minimum concentration required.

^※Data from a previous publication ²⁷.