Circular representation of IPAV chromosomes with predicted CDSs. Left, large chromosome (CI); right, small chromosome (CII). The outer scales are numbered in megabases from the origins of replication (Ori). Circles range from 1 (outer circle) to 7 (inner circle) for CI and from I (outer circle) to V (inner circle) for CII. Circle 1, representation of genome structure when compared with L. interrogans strain Fiocruz L1-130 (yellow, the collinear region; blue, the large inverse region). Circle 2/I and 3/II, genes on plus and minus strands (colors representing functional categories according to COGs). Circle 4, tRNA genes (red) and rRNA genes (blue). Circle 5/III, IS elements (both intact and remnant are included; green, ISlin1; black, other types of ISs). Circle 6/IV, GC skew (calculated using a 2 500 bp (CI)/500 bp (CII) window sliding 200 bp (CI)/100 bp (CII) at a time; red, values > 0; green, values < 0). Circle 7/V, GC content.