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. 2011 Apr 12;21(7):1131–1142. doi: 10.1038/cr.2011.64

Figure 4.

Figure 4

XerR relieves its inhibition on xccR expression in planta. (A) In planta cultivation did not significantly increase the GUS activity from Xcc 8099, while it had the opposite influence on that of Xcc 8099/pFR423 and Xcc 8177/pFR423. The bacteria were recovered from vacuum-infiltrated cabbage leaves 30 h post infiltration, and GUS activities were assayed. Data and standard deviation represented the mean of three independent measurements. (B and C) Plant signal(s) alleviated the binding activity of XerR protein to the xccR upstream regulatory sequence. EMSA assays with biotin-labeled probe were performed by MBP-XerR with plant extracts (< 1 kDa) at two dilutions.