Figure 2.

Levels of messenger RNA for MMP genes were determined by real-time reverse-transcription polymerase chain reaction in five pairs of donor age–matched human dermal fibroblast lines. Symbols (○ = controls; ▲ = Hutchinson–Gilford progeria syndrome) indicate mean relative MMP-3 (A), -2 (B), and -9 (C) RNA levels standardized to β-actin for two separate experiments analyzed in duplicate; solid lines indicate the mixed linear model’s predicted mean with dashed lines representing upper and lower 95% confidence intervals.