Figure 3.

MMP-3 secretion (A and B) was determined in five donor age–matched pairs of human dermal fibroblast lines from 24-hour conditioned medium utilizing an enzyme-linked immunosorbent assay (n = 3 experiments per pair). (A) Bars (white = control; black = Hutchinson–Gilford progeria syndrome [HGPS]) indicate mean MMP-3 secretion of all five fibroblast lines per group, and error bars indicate upper and lower 95% confidence intervals. (B) Symbols (○ = controls; ▲= HGPS) indicate mean secreted protein levels from three replicate experiments for each individual cell line; solid lines indicate the mixed linear model’s predicted mean with dashed lines representing upper and lower 95% confidence intervals. Gelatin zymogram (C) of two representative samples of 24-hour conditioned media normalized to total cellular protein from control and HGPS cells. Enzyme levels of MMP-2 (D) and MMP-9 (E) were scored based on band intensity. Bars (white = control; black = HGPS) indicate means of all five fibroblast lines per group with standard errors (n = 2 experiments per pair).