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. 2011 Oct 14;6(10):e26065. doi: 10.1371/journal.pone.0026065

Figure 2. Calcium influx, activation of D1 receptors, and induction of the cAMP-PKA cascade are all required for the induction and maintenance of LTP in BLA principal neurons.

Figure 2

A, B) Inclusion of the calcium chelator, BAPTA (10 mM) in the patch pipette, fully blocked the induction of LTP by either TBS (n = 6) or 5xHFS (n = 5). C, D) Endogenous dopamine was released during TBS and 5xHFS and was necessary for the induction of LTP in BLA principal neurons. Incubation with SCH23390 (10 µM) before and during TBS (n = 6) or 5xHFS (n = 6) completely abolished the induction of LTP. E, F) The induction LTP by either TBS or 5xHFS was dependent on activation of the cAMP-PKA signaling cascade. Intracellular application of PKA inhibitor KT5720 (100 nM) fully blocked TBS-induced LTP (n = 5), as well as 5xHFS-induced LTP (n = 5).