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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Cancer Res. 2011 Aug 25;71(20):6475–6484. doi: 10.1158/0008-5472.CAN-11-1702

Figure 2.

Figure 2

KLF5 and p53 bind and coordinately regulate NOTCH1. (A-B) As expected, quantitative chromatin immunoprecipitation (ChIP) revealed binding of p53 (A) to NOTCH1. However, when p53 binding was lost, with p53R175H or p53 shRNA, there was a reciprocal rise in KLF5 binding (B) to the same region. (C-D) NOTCH1 luciferase reporter (N1PR) was activated by KLF5 (C) and inhibited by KLF5 suppression (D) in EPC2-hTERT-p53R175H cells. Mutations within the p53 binding sites of N1PR (N1PRmt) failed to abolish these effects, but NOTCH1 responsiveness to KLF5 was abolished by additional mutations in two putative KLF5 binding sites.