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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Cancer Res. 2011 Aug 25;71(20):6475–6484. doi: 10.1158/0008-5472.CAN-11-1702

Figure 3.

Figure 3

KLF5 knockdown with p53R175H promotes keratinocyte transformation, tumor growth, and invasion. (A). By 14 days, numerous cells with both KLF5 knockdown and p53R175H became spindle-shaped (arrows), compared to the rounded, epithelioid appearance with KLF5 knockdown alone, consistent with a mesenchymal phenotype and EMT. (B) Quantification revealed a statistically significant difference in the number of spindle shaped cells with both KLF5 knockdown and p53R175H, compared to KLF5 knockdown or p53R175H alone. (C) Growth in soft agar was seen with both KLF5 knockdown and p53R175H but not with either KLF5 knockdown or p53R175H alone. Restoration of Notch signaling with NICD inhibited colony formation in soft agar of EPC2-hTERT cells with KLF5 knockdown and p53R175H.