Inhibitory effect of 30 μM NS1619 on 0.1 μM carbachol-induced phasic and tonic contractions of rat DSM isolated strips. A) A representative trace from a DSM isolated strip and the effect of NS1619 on carbachol-induced contractions. Summary data illustrating that NS1619 caused a significant decrease in the amplitude, muscle force integral, frequency and tone. B) NS1619 (30 μM) decreased the amplitude, muscle force integral, frequency and tone of carbachol-induced contractions. 0.1 μM carbachol-induced contractions were taken to be 100%. n=9, N=7, **P<0.005; *P<0.05. Data are means ± S.E.M.