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Glutamic acid decarboxylase (GAD65/67)-immunopositive cells in the white matter are neurons and not astrocytes, as determined by double-label immunocytochemistry with the neuronal marker microtubule-associated protein 2 (MAP2) and the astrocytic marker glial fibrillary acidic protein (GFAP), respectively. (A) A unipolar MAP2-immunopositive neuron co-expresses GAD65/67. (B) Two granular GABAergic neurons (arrows) are immunopositive for GAD65/67 but negative for GFAP (arrowheads).
