Table 2.
Antibodies
Antibody | Host | Catalog No./Source | Dilution | Labels | References |
---|---|---|---|---|---|
GAD67/65 | Rabbit Polyclonal IgG | ab49832/Abcam, Cambridge, MA | 1:1000 and 1:10,000 | Brain GAD 65 and GAD 67 isoforms | (30) |
MAP2 | Mouse Monoclonal IgG | SMI-52R/Covance, Dedham, MA | 1:800 | MAP2a and b and a doublet at 68 kDa that may represent MAP2c | (31) |
GFAP | Mouse Monoclonal IgG | SMI-22/Covance | 1:500 | Astrocytes and Bergmann glia | (32) |
Double-cortin (C18) | Goat polyclonal IgG | SC8066/Santa Cruz Biotechnology, Santa Cruz, CA | 1:100 | Doublecortin | (33) |
Active caspase-3 | Rabbit Monoclonal IgG | 559565/BD Biosciences, Franklin Lakes, NJ | 1:20,000 | Active form of Caspase-3 | (34) |
GABAA (α1) | Rabbit Polyclonal IgG | AGA001/Alomone, Jerusalem, Israel | 1:200 | GABA(A) receptor α1 | (29) |
GABAA (α3) | Rabbit Polyclonal IgG | AGA003/Alomone | 1:200 | GABA(A) receptor α3 | (29) |
GABA, γ-aminobutyric acid; GAD, glutamic acid decarboyxlase; GFAP, glial fibrillary acidic protein; MAP2, microtubule-associated protein-2