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. Author manuscript; available in PMC: 2012 Aug 16.
Published in final edited form as: Oncogene. 2011 Jul 11;31(7):828–841. doi: 10.1038/onc.2011.277

FIGURE 3. MDM2 genetic inactivation triggers melanoma growth arrest and senescence.

FIGURE 3

A,B, Protein immunoblots of the indicated cell lines that were either uninfected (−) or infected for GFP-lentiviruses coding for Control shRNA (Ctrl) or MDM2 shRNA. C, (Left panels) Representative brightfield and corresponding GFP fluorescent microphotographs (day 7) of SK-Mel-103 transduced with the indicated lentiviral vectors. (Middle panels) Cell cycle distribution as determined by flow cytometry (day 3). (Right panels) Visualization of p53 induction by immunofluorescence. D, SA-β-Gal staining in MDM2 shRNA-expressing SK-Mel-103 cells as compared to uninfected and Control shRNA cells. E, Corresponding quantification of cells exhibiting positive blue staining for SA-β-Gal. Data correspond to the mean ± SEM of cells from three experiments.