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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Carcinog. 2011 Jul 7;51(6):465–474. doi: 10.1002/mc.20811

Figure 2.

Figure 2

The PEITC treatment caused apoptosis in BRI-JM04 cells. (A) Representative microscopic images depicting apoptotic cells with condensed and fragmented DNA (DAPI assay) in BRI-JM04 cultures after 48-hour treatment with DMSO (control) or the indicated concentrations of PEITC. (B) Quantitation of apoptotic cells (DAPI assay) in BRI-JM04 cultures after 24- or 36-hour treatment with DMSO (control) or the indicated concentrations of PEITC. Results are expressed as enrichment factor relative to DMSO-treated control. Experiment was repeated twice in triplicate and combined data from both experiments are shown as mean ± SD (n = 6). **Significantly different (P<0.01) compared with corresponding control by one-way ANOVA with Dunnett’s adjustment. (C) Western blotting for cleaved caspase-3 using lysates from BRI-JM04 cells after 24- or 36-hour treatment with DMSO (control) or the indicated concentrations of PEITC. Numbers above bands represent changes in levels of cleaved caspase-3 relative to DMSO-treated control at each time point.