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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Carcinog. 2011 Jul 7;51(6):465–474. doi: 10.1002/mc.20811

Figure 6.

Figure 6

RNA interference of Bim partially protected against PEITC-induced apoptosis in human breast cancer cells. (A) Western blotting for BimEL using lysates from MCF-7 and MDA-MB-231 cells transiently transfected with a control siRNA (lane 1) or a Bim-targeted siRNA (lane 2). (B) Quantitation of apoptotic cells (DAPI assay) in MCF-7 and MDA-MB-231 cells transiently transfected with a control siRNA or a Bim-targeted siRNA and treated for 48 hours with DMSO or the indicated concentrations of PEITC. Results are expressed as enrichment factor relative to corresponding DMSO-treated control siRNA transfected cells (mean ± SD; n = 3). Significantly different (P<0.05) compared with acorresponding DMSO-treated control, and bbetween PEITC-treated groups by one-way ANOVA followed by Bonferroni’s test.