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Spectroscopic characterization of 2′-azido-modified oligoribonucleotides. A) Overlay of UV melting profiles of GAAGGGCAACCUUCG, GAAGGGCAACCU*UCG, GAA*GGGCAACCUUCG, cRNA=5 μm; B) Overlay of CD spectra of GAAGGGCAACCUUCG, GAAGGGCAACCU*UCG, GAA*GGGCAACCUUCG, cRNA=2 μm. Conditions: 10 mm Na2HPO4, 150 mm NaCl, pH 7.0. U*=2′-azido-2′-deoxyuridine, A*=2′-azido-2′-deoxyadenosine, H=hyperchromicity.
