Skip to main content
. 2010 Dec 17;12(1):47–51. doi: 10.1002/cbic.201000646

Figure 3.

Figure 3

Attachment of a fluorescence quencher to RNA with an internal site-specific ribose 2′-azido group. A) Concept of molecular beacon (MB); B) Staudinger ligation of the phosphine derivative 10 to RNA 11; C) Timedependent fluorescence response of the MB 12 to addition of the target sequence 13 (left) and of the near-cognate sequence 14 (right) with double mismatch discrimination (mismatched nucleobases underlined). Conditions: cMB=0.5 μm, ctarget=2.5 μm, 100 mm MES⋅NaOH, 1.0 mm MgCl2, pH 6.5. λex=490 nm, λem=514 nm. f=fluorescence (emission), t=time.