Fig. 2.

Reactive oxygen species (ROS) production by polymorphonuclear neutrophils (PMN) as a function of CD4⁺CD25⁺CD39⁺. ROS production in 27 children with active nephrotic syndrome. All patients presented proteinuria at the time of the analysis (proteinuria > 7 g/24 h in 11, 1–5 g/24 h in 5, 0·5–1/g 24 h in 11). Only nine had a pathology diagnosis [focal segmental glomerulosclerosis (FSGS) four cases, minimal change nephrosis (MCN) four cases, immunoglobulin (Ig)M one]. (a) ROS production as a function of CD4⁺CD25⁺CD39⁺ in children with idiopathic nephrotic syndrome (iNS) (open squares) and effects of exogenous apyrase (closed circles); (b) ROS production in iNS patients with different percentage of CD4⁺CD25⁺CD39⁺cells and modification produced by the addition of apyrase in vitro; (c) ROS production as a function of CD4⁺CD25⁺CD39⁺ in normal subjects (open squares) and effects of exogenous apyrase (closed circles); (d) ROS production in normal subjects with different percentage of CD4⁺CD25⁺CD39⁺cells and modification produced by the addition of apyrase in vitro. **P < 0·005; *P < 0·05.