Fig. 1.

Gating strategy. CD3⁺ lymphocytes were selected on sidewards scatter (SSC) and CD3⁺ expression. T cells were divided for both CD4⁺ and CD8⁺ expression. Both subtypes were typed further for CD27 and CD45RO expression. CD27⁺CD45RO^- are naive, CD27⁺CD45RO⁺ are central memory, CD27^-CD45RO⁺ are effector memory and CD27^-CD45RO^- are terminally differentiated T cells. All subtypes were analysed for CCR4⁺ and CCR4⁺⁺ expression.