Effects of channel blockers, AP-5, TTX, and Ni2+. a A schematic drawing of the experiment. Type 3 paired stimuli were applied to the proximal area at Δd = 5 or 10 μm. b The effects of channel blocker application at Δd = 5 μm. Black and gray lines indicate the traces for paired stimuli (Type 3) and the linear summation of individual inputs. c The graphs summarize the effects of channel blocker application at Δd = 5 and 10 μm. The Y-axis indicates the supralinear amplification ratio (Δα, see text). d The time constant ratio (Δτ, see text) at Δd = 5 and 10 μm. Error bars indicate the standard error of the mean and asterisks indicate the level of significance: * P < 0.05 and ** P < 0.01