Fig. 5.

Calculation of the PCSs for the bound state of the pYTN tripeptide. a Selected region of the ¹H NMR spectra of the pYTN tripeptide, acquired during titration of LBT-Grb2 containing Tm³⁺. The spectra of the tripeptide in the presence of 0 equivalent (blue) to 0.7 equivalent (purple) LBT-Grb2-Tm³⁺ are overlaid. b Chemical shift changes of the signals of the pYTN tripeptide during titration with increasing amounts LBT-Grb2 containing Tm³⁺. c The chemical structure of the pYTN tripeptide on which the observed PCS values are indicated