Fig. 7.

Analysis on the high-affinity inhibitor and LBT-Grb2. a The chemical structure of the inhibitor in which the protons were labeled. Proton signals were assigned according to the previous work (Ogura et al. 2008). Observed PCS values are indicated. b Overlay of the ¹H 1D spectra of the high-affinity inhibitor complexed with ²H¹⁵N-labeled LBT-Grb2. The spectra measured in the presence and absence of the paramagnetic lanthanide ion Tm³⁺ are shown in blue and gray, respectively. An asterisk indicates an impurity. Spectra were acquired using an 800 MHz NMR spectrometer at 25°C