Figure 4. Tet2 deletion leads to progressive defects in bone marrow and extramedullary hematopoiesis.

A) FACS analysis of bone marrow stem and progenitor populations of Tet2^−/− (vav-Cre⁺Tet2^f/f) and WT (vav-Cre⁺Tet2^wt/wt) at two different ages (4–6 and 20 weeks). Antibody stainings are as indicated. B) Identical antibody labeling as in A) but using spleen cells from Tet2^−/− (vav-Cre⁺Tet2^f/f) and WT (vav-Cre⁺Tet2^wt/wt) mice. C) Absolute numbers of spleen LSK cells in Tet2^−/− (vav-Cre⁺Tet2^f/f) and WT (vav-Cre⁺Tet2^wt/wt) mice at two different ages. P values are show for each comparison. For all experiments shown in this figure n=8 for each genotype. Error bars represent ± SD. See also Figure S3.