Figure 4. Y2H assay shows that oligomerization of DEG7 is mediated by the second (degenerated) protease domain.

Growth of S. cerevisiae YRG2 cells on histidine-free medium (indicative for interaction) is only visible when the combination of interaction partners contains the second (degenerated) protease domain (deg. prot. dom.), which is part of the second half (2nd half) and the full-length protein (fl). No interaction occurs with constructs containing only the first half (1st half) or first (active) protease domain (act. prot. dom.). The columns show different dilutions of the yeast cultures. A dilution factor of 1×corresponds to a D₆₀₀ of 1.0. Controls: neg., negative; pos., positive.