Table 1. Interaction of DEG7 domains assayed by Y2H.
Abbreviations: +/+, HIS3 reporter gene activation with both possible AD- and BD-fusion combinations; +/−, HIS3 reporter gene activation with one combination only, −/− no HIS3 reporter gene activation with either possible combination. Interacting domains are marked in bold.
| Constructs | Interaction |
|---|---|
| DEG7-fl/DEG7-fl | +/+ |
| DEG7-fl/first half | +/− |
| DEG7-fl/second half | +/+ |
| DEG7-fl/active protease domain | +/− |
| DEG7-fl/degenerated protease domain | +/− |
| DEG7-fl/PDZ1 | −/− |
| DEG7-fl/PDZ3+4 | −/− |
| DEG7-fl/empty | −/− |
| First half/first half | −/− |
| First half/second half | +/+ |
| First half/active protease domain | −/− |
| First half/degenerated protease domain | +/− |
| First half/PDZ1 | −/− |
| First half/PDZ3+4 | −/− |
| First half/empty | −/− |
| Second half/second half | +/+ |
| Second half/active protease domain | +/− |
| Second half/degenerated protease domain | +/+ |
| Second half/PDZ1 | −/− |
| Second half/PDZ3+4 | −/− |
| Second half/empty | −/− |
| Active protease domain/active protease domain | −/− |
| Active protease domain/degenerated protease domain | +/− |
| Active protease domain/PDZ1 | −/− |
| Active protease domain/PDZ3+4 | −/− |
| Active protease domain/empty | −/− |
| Degenerated protease domain/degenerated protease domain | +/+ |
| Degenerated protease domain/PDZ1 | −/− |
| Degenerated protease domain/PDZ3+4 | −/− |
| Degenerated protease domain/empty | −/− |
| PDZ1/PDZ1 | −/− |
| PDZ1/PDZ3+4 | −/− |
| PDZ1/empty | −/− |
| PDZ3+4/PDZ3+4 | −/− |
| PDZ3+4/empty | −/− |