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. 2011 Sep 21;8(9):3764–3776. doi: 10.3390/ijerph8093764

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© 2011 by the authors; licensee MDPI, Basel, Switzerland

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Isolation of LF and Lp in a stepwise manner from the defatted colostrum using SPEC 70 SLS column (Φ 1.6 × 6 cm) after static adsorption and the analysis graph obtained from Quantity One software. SDS-PAGE: Lane 1, standard protein markers; Lane 2, Lf; Lane 3, Lp; Lane 4, IgG; Lanes 5 and 6, the isolates of first and second peaks eluted by different concentrations of NaCl.